Gut adhesive Bacillus subtilis spores as a platform for the 1 mucosal delivery of antigens
نویسندگان
چکیده
8 Vaccine Development Laboratory, Department of Microbiology, Institute of Biomedical 9 Sciences, University of São Paulo, São Paulo, Brazil. 10 Vaccine Development Laboratory, Department of Parasitology, Institute of Biomedical 11 Sciences, University of São Paulo, São Paulo, Brazil 12 Cancer Genomics Group, International Centre for Genetic Engineering and 13 Biotechnology (ICGEB), Cape Town 7925, South Africa. 14
منابع مشابه
Gut adhesive Bacillus subtilis spores as a platform for mucosal delivery of antigens.
Bacillus subtilis spores have been used as safe and heat-resistant antigen delivery vectors. Nonetheless, the oral administration of spores typically induces weak immune responses to the passenger antigens, which may be attributed to the fast transit through the gastrointestinal tract. To overcome this limitation, we have developed B. subtilis spores capable of binding to the gut epithelium by ...
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Bacterial spores spontaneously interact and tightly bind heterologous proteins. A variety of antigens and enzymes have been efficiently displayed on spores of Bacillus subtilis, the model system for spore formers. Adsorption on B. subtilis spores has then been proposed as a non-recombinant approach for the development of mucosal vaccine/drug delivery vehicles, biocatalysts, bioremediation, and ...
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Development of mucosal vaccines strongly relies on an efficient delivery system and, over the years, a variety of approaches based on phages, bacteria or synthetic nanoparticles have been proposed to display and deliver antigens. The spore of Bacillus subtilis displaying heterologous antigens has also been considered as a mucosal vaccine vehicle, and shown able to conjugate some advantages of l...
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BACKGROUND Mucosal infections are a major global health problem and it is generally accepted that mucosal vaccination strategies, able to block infection at their entry site, would be preferable with respect to other prevention approaches. However, there are still relatively few mucosal vaccines available, mainly because of the lack of efficient delivery systems and of mucosal adjuvants. Recomb...
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Tuberculosis (TB) is the most deadly infectious disease in existence, and the only available vaccine, Bacillus Calmette-Guérin (BCG), is almost a century old and poorly protective. The immunological complexity of TB, coupled with rising resistance to antimicrobial therapies, necessitates a pipeline of diverse novel vaccines. Here, we show that Bacillus subtilis spores can be coated with a fusio...
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تاریخ انتشار 2014